7YGW
Crystal structure of the Zn2+-bound EFhd1/Swiprosin-2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-06-03 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.28255 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.959, 47.393, 63.399 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.960 - 1.720 |
R-factor | 0.1992 |
Rwork | 0.198 |
R-free | 0.21050 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7clt |
RMSD bond length | 0.009 |
RMSD bond angle | 0.984 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.20.1-4487) |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.750 |
High resolution limit [Å] | 1.720 | 1.720 |
Rmerge | 0.079 | |
Number of reflections | 14577 | 685 |
<I/σ(I)> | 24.4 | |
Completeness [%] | 99.5 | 94.5 |
Redundancy | 12.5 | |
CC(1/2) | 1.000 | 0.774 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1 M Tris-HCl pH 8.5, 2.5 mM ZnSO4, 25% (w/v) Jeffamine ED-2001 |