7YGV
Crystal structure of the Ca2+-bound EFhd1/Swiprosin-2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-11-03 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.28255 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.251, 47.907, 63.434 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.720 - 2.800 |
R-factor | 0.2004 |
Rwork | 0.195 |
R-free | 0.25200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7clt |
RMSD bond length | 0.009 |
RMSD bond angle | 1.039 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.20.1-4487) |
Refinement software | PHENIX (1.20.1-4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.850 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.492 | |
Rmeas | 0.517 | |
Rpim | 0.150 | |
Number of reflections | 3596 | 176 |
<I/σ(I)> | 20 | 3.7 |
Completeness [%] | 99.8 | |
Redundancy | 22 | |
CC(1/2) | 0.999 | 0.959 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1 M Tris-HCl pH 8.5, 0.4 mM ZnSO4, and 25% (w/v) Jeffamine ED-2001 |