7YGK

Crystal structure of a secretory phospholipase A2 from Sciscionella marina

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-15
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	47.380, 39.680, 54.300
Unit cell angles	90.00, 91.55, 90.00

Refinement procedure

Resolution	27.140 - 1.240
R-factor	0.17661699011
Rwork	0.175
R-free	0.19716
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1faz
RMSD bond length	0.015
RMSD bond angle	1.302
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.290
High resolution limit [Å]	1.240	1.240
Number of reflections	28410	2762
<I/σ(I)>	15.7
Completeness [%]	99.1
Redundancy	6.4
CC(1/2)	0.995	0.995

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	5.5	295.15	100 mM citrate pH 5.5, 40% (v/v) PEG 600