7YFV
Structure of Rpgrip1l CC1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-11-08 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97915 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 35.167, 104.674, 38.413 |
Unit cell angles | 90.00, 105.08, 90.00 |
Refinement procedure
Resolution | 29.100 - 2.200 |
R-factor | 0.2163 |
Rwork | 0.214 |
R-free | 0.26310 |
Structure solution method | AB INITIO PHASING |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | Arcimboldo |
Refinement software | PHENIX (1.18.2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.240 |
High resolution limit [Å] | 2.200 | 5.970 | 2.200 |
Rmerge | 0.074 | 0.030 | 0.807 |
Rmeas | 0.080 | 0.033 | 0.874 |
Rpim | 0.030 | 0.012 | 0.332 |
Total number of observations | 94590 | ||
Number of reflections | 13799 | 707 | 690 |
<I/σ(I)> | 4.2 | ||
Completeness [%] | 99.6 | 99 | 99.3 |
Redundancy | 6.9 | 6.4 | 6.7 |
CC(1/2) | 0.999 | 0.786 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 289 | 7.5% w/v PEG6000, 0.1 M Sodium citrate tribasic dihydrate, pH 4.0 |