7YFU
Structure of Rpgrip1l CC2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-01-08 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97915 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 81.620, 26.939, 37.469 |
Unit cell angles | 90.00, 90.69, 90.00 |
Refinement procedure
Resolution | 21.160 - 1.500 |
R-factor | 0.1791 |
Rwork | 0.177 |
R-free | 0.21540 |
Structure solution method | AB INITIO PHASING |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | Arcimboldo |
Refinement software | PHENIX (1.18.2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 1.530 |
High resolution limit [Å] | 1.500 | 4.070 | 1.500 |
Rmerge | 0.065 | 0.048 | 0.336 |
Rmeas | 0.071 | 0.053 | 0.383 |
Rpim | 0.029 | 0.022 | 0.177 |
Total number of observations | 77240 | ||
Number of reflections | 13124 | 709 | 551 |
<I/σ(I)> | 9.8 | ||
Completeness [%] | 98.7 | 99.4 | 85.2 |
Redundancy | 5.9 | 5.7 | 4.1 |
CC(1/2) | 0.997 | 0.958 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 289 | 2.0 M ammonium sulfate |