7YF4

Crystal structure of METTL9 in complex with SLC39A5 mutant peptide and SAH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97915
Spacegroup name	C 1 2 1
Unit cell lengths	142.387, 43.213, 106.231
Unit cell angles	90.00, 122.77, 90.00

Refinement procedure

Resolution	59.860 - 2.750
R-factor	0.229
Rwork	0.223
R-free	0.28530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7yf2
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.860	2.900
High resolution limit [Å]	2.750	2.750
Number of reflections	14118	14118
<I/σ(I)>	9.9
Completeness [%]	97.3
Redundancy	5.9
CC(1/2)	0.997	0.894

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M MES monohydrate pH6.5, 12% w/v PEG 20000, 0.2 M sodium thiocyanate