7YF3
Crystal structure of METTL9 in complex with unmethylated S100A9 peptide and SAH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 141.892, 43.547, 106.492 |
| Unit cell angles | 90.00, 123.70, 90.00 |
Refinement procedure
| Resolution | 70.319 - 3.434 |
| R-factor | 0.2407 |
| Rwork | 0.236 |
| R-free | 0.28810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7yf2 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.320 | 3.640 |
| High resolution limit [Å] | 3.430 | 3.430 |
| Number of reflections | 7067 | 7067 |
| <I/σ(I)> | 5.8 | |
| Completeness [%] | 94.7 | |
| Redundancy | 6.3 | |
| CC(1/2) | 0.984 | 0.797 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M Tris pH 8.5, 0.2 M MgCl2, 30% (w/v) PEG 4000, 0.1 M NDSB-256 |
