7YEW
Structure of MmIGF2BP3 in complex with 7-mer RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-09-21 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97915 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 45.890, 73.716, 57.405 |
Unit cell angles | 90.00, 101.80, 90.00 |
Refinement procedure
Resolution | 28.490 - 2.500 |
R-factor | 0.1978 |
Rwork | 0.195 |
R-free | 0.25250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6fqr |
RMSD bond length | 0.009 |
RMSD bond angle | 1.131 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.146 | 0.872 |
Rmeas | 0.154 | 0.960 |
Rpim | 0.058 | 0.395 |
Number of reflections | 12566 | 1264 |
<I/σ(I)> | 13.69 | 2 |
Completeness [%] | 96.5 | 97.2 |
Redundancy | 6.6 | 5.4 |
CC(1/2) | 0.992 | 0.676 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.2 M Ammonium acetate, 0.1 M Sodium citrate tribasic dihydrate pH 5.6, 30% w/v Polyethylene glycol 4000 |