7YD4
Crystal structure of an N terminal truncated secreted protein, Rv0398c from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 40.881, 73.463, 103.724 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.975 - 1.896 |
| R-factor | 0.1895 |
| Rwork | 0.185 |
| R-free | 0.22940 |
| Structure solution method | AB INITIO PHASING |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.8) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.975 | 1.940 |
| High resolution limit [Å] | 1.890 | 1.900 |
| Rmerge | 0.119 | 0.889 |
| Rmeas | 0.128 | 0.950 |
| Rpim | 0.045 | 0.341 |
| Number of reflections | 12738 | 1255 |
| <I/σ(I)> | 12.2 | |
| Completeness [%] | 99.5 | 96.6 |
| Redundancy | 7.8 | 7.6 |
| CC(1/2) | 0.998 | 0.788 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | Ammonium sulphate, Sodium potassium tartrate, Trisodium citrate, pH 5.6 |
