7YCV

The Dimeric Format of Truncated PrpA (2-54)and RHH Domain of PrpA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-17
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979183
Spacegroup name	I 4 2 2
Unit cell lengths	83.252, 83.252, 70.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.340 - 2.610
R-factor	0.2297
Rwork	0.228
R-free	0.27140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7b22
RMSD bond length	0.012
RMSD bond angle	1.444
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.340	2.706
High resolution limit [Å]	2.610	2.612
Rmerge	0.104	0.851
Rmeas	0.108	0.884
Rpim	0.029	0.231
Number of reflections	3976	373
<I/σ(I)>	16.46	2.99
Completeness [%]	99.3	97.9
Redundancy	13.7	13.3
CC(1/2)	0.998	0.907

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	289	0.2 M Magnesium chloride hexahydrate, 0.1 M HEPES sodium pH 7.5, 30 % v/v PEG 400