7YCO

Crystal structure of SARS-CoV-2 Receptor Binding Domain bound to A6 repebody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-19
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0
Spacegroup name	I 41
Unit cell lengths	135.966, 135.966, 69.556
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.800 - 1.960
R-factor	0.2362
Rwork	0.235
R-free	0.25850
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.080
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.800	2.030
High resolution limit [Å]	1.960	1.960
Rmerge	0.085	1.972
Number of reflections	45636	4541
<I/σ(I)>	19.88	1.52
Completeness [%]	99.9	99.98
Redundancy	13.5	14.2
CC(1/2)	0.999	0.744

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M sodium citrate tribasic dihydrate, 20 % (w/v) PEG 3350