7YC2
Crystal structure of auxiliary protein in complex with human protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17B1 |
Synchrotron site | SSRF |
Beamline | BL17B1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-05-01 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9785 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 106.300, 72.800, 127.500 |
Unit cell angles | 90.00, 113.40, 90.00 |
Refinement procedure
Resolution | 17.050 - 2.900 |
R-factor | 0.2504 |
Rwork | 0.250 |
R-free | 0.26230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7ep0 |
Data reduction software | XDS |
Data scaling software | XDS |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.650 | 3.070 |
High resolution limit [Å] | 2.890 | 2.890 |
Rmerge | 0.270 | |
Number of reflections | 19906 | 6197 |
<I/σ(I)> | 4.13 | |
Completeness [%] | 98.4 | 98.2 |
Redundancy | 3.36 | |
CC(1/2) | 0.120 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 296 | 1.4 M Sodium phosphate monobasic monohydrate/Potassium phosphate dibasic |