7YBD
Crystal structure of sliding DNA clamp of Clostridioides difficile
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-05 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 82.899, 65.233, 81.994 |
| Unit cell angles | 90.00, 118.11, 90.00 |
Refinement procedure
| Resolution | 41.452 - 2.130 |
| Rwork | 0.179 |
| R-free | 0.23920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.519 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238 2018/15/10) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.680 | 2.190 |
| High resolution limit [Å] | 2.130 | 2.130 |
| Rmerge | 0.066 | 0.608 |
| Rmeas | 0.079 | 0.718 |
| Number of reflections | 21383 | 1733 |
| <I/σ(I)> | 11.9 | 2.2 |
| Completeness [%] | 98.7 | 98.2 |
| Redundancy | 3.4 | 3.5 |
| CC(1/2) | 0.997 | 0.769 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.1 M HEPES-NaOH pH 7.5, 0.2 M magnesium formate, and 18% PEG3350 |
