7YAZ
Crystal structure of ZAK in complex with compound YH-186
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-21 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 129.976, 48.608, 42.881 |
| Unit cell angles | 90.00, 105.78, 90.00 |
Refinement procedure
| Resolution | 31.270 - 2.540 |
| R-factor | 0.1866 |
| Rwork | 0.183 |
| R-free | 0.25180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5x5o |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.540 | 2.550 |
| Rpim | 0.107 | 0.252 |
| Number of reflections | 8584 | 829 |
| <I/σ(I)> | 16 | 2 |
| Completeness [%] | 99.8 | |
| Redundancy | 17 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.05 M Magnesium acetate, 0.05 M Sodium acetate pH 5.0, 28% PEG400 |
