7YAX
HYDROXYNITRILE LYASE FROM THE MILLIPEDE,
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2017-07-05 |
| Detector | RIGAKU |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 63 |
| Unit cell lengths | 123.630, 123.630, 129.130 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.969 - 2.010 |
| Rwork | 0.145 |
| R-free | 0.17950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6kfe |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.570 | 2.050 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmerge | 0.061 | 0.221 |
| Number of reflections | 595005 | 4547 |
| <I/σ(I)> | 20.6 | |
| Completeness [%] | 99.6 | |
| Redundancy | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.1 M BIS-TRIS (pH 5.5), 2.0 M ammonium sulfate |
