7YAO
Crystal structure of a bright green fluorescent protein (oxStayGold) in jellyfish Cytaeis uchidae from Biortus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-06-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.18058 |
| Spacegroup name | P 61 |
| Unit cell lengths | 132.107, 132.107, 58.887 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.960 - 1.500 |
| Rwork | 0.160 |
| R-free | 0.17770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AF2 predicted |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.587 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.960 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.072 | 0.888 |
| Number of reflections | 93826 | 4614 |
| <I/σ(I)> | 20.6 | |
| Completeness [%] | 100.0 | |
| Redundancy | 9.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2.1M DL-Malic acid pH7.0 |
