7YA8
The crystal structure of IpaH2.5 LRR domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.785, 137.686, 163.563 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.400 |
| R-factor | 0.20039 |
| Rwork | 0.198 |
| R-free | 0.24494 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7ya7 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.677 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.419 |
| High resolution limit [Å] | 3.300 | 3.301 |
| Rmerge | 0.152 | 0.053 |
| Number of reflections | 22003 | 1436 |
| <I/σ(I)> | 22 | 4.9 |
| Completeness [%] | 96.0 | 100 |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.1 M succinic acid pH 6.6, 18.8% (w/v) polyethylene glycol (PEG)3350 |
