7YA7
The crystal structure of IpaH1.4 LRR domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-05-17 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.9 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 33.467, 68.039, 100.260 |
Unit cell angles | 90.00, 100.72, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.400 |
R-factor | 0.18712 |
Rwork | 0.185 |
R-free | 0.21984 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5b0n |
RMSD bond length | 0.029 |
RMSD bond angle | 2.502 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.420 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.062 | 0.487 |
Number of reflections | 86206 | 8222 |
<I/σ(I)> | 33.9 | 4 |
Completeness [%] | 98.6 | |
Redundancy | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 50 mM imidazole (pH 8.0), 1.2 M NaCl. |