7Y9B
Crystal structure of the membrane (M) protein of a SARS-COV-2-related coronavirus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-09-19 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97915 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 76.569, 66.571, 112.628 |
Unit cell angles | 90.00, 109.83, 90.00 |
Refinement procedure
Resolution | 37.090 - 3.214 |
R-factor | 0.2719 |
Rwork | 0.271 |
R-free | 0.29310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7y96 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.412 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.878 | 48.878 | 3.252 |
High resolution limit [Å] | 3.194 | 8.904 | 3.194 |
Rmerge | 0.147 | 0.035 | 0.697 |
Rmeas | 0.175 | 0.043 | 0.835 |
Rpim | 0.094 | 0.025 | 0.453 |
Total number of observations | 56567 | 2580 | 2692 |
Number of reflections | 16908 | 844 | 846 |
<I/σ(I)> | 6.9 | 19.3 | 1.6 |
Completeness [%] | 94.1 | 95.6 | 91.9 |
Redundancy | 3.3 | 3.1 | 3.2 |
CC(1/2) | 0.994 | 0.998 | 0.764 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 8.8 | 293 | 300 mM ammonium formate, 50 mM Tris-HCl pH 8.8, 35% PEG 500 MME, 7.1 mM pentaethylene glycol monooctyl ether |