7Y8S
Crystal structure of sDscam FNIII1-3 domains, isoform beta2v6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-12-12 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97855 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 171.649, 171.649, 114.933 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.202 - 2.696 |
R-factor | 0.2245 |
Rwork | 0.222 |
R-free | 0.27450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1va9 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.688 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.15.2_3472: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.750 |
High resolution limit [Å] | 2.696 | 7.320 | 2.700 |
Rmerge | 0.226 | 0.087 | 2.131 |
Rmeas | 0.229 | 0.089 | 2.166 |
Rpim | 0.037 | 0.016 | 0.382 |
Number of reflections | 28081 | 1575 | 1383 |
<I/σ(I)> | 3.4 | ||
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 38.3 | 35.5 | 31.8 |
CC(1/2) | 0.981 | 0.968 | 0.778 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 20% (w/v) PEG 3,350, 100 mM PCB buffer pH 4.5 |