7Y8S

Crystal structure of sDscam FNIII1-3 domains, isoform beta2v6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97855
Spacegroup name	P 64 2 2
Unit cell lengths	171.649, 171.649, 114.933
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.202 - 2.696
R-factor	0.2245
Rwork	0.222
R-free	0.27450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1va9
RMSD bond length	0.004
RMSD bond angle	0.688
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.750
High resolution limit [Å]	2.696	7.320	2.700
Rmerge	0.226	0.087	2.131
Rmeas	0.229	0.089	2.166
Rpim	0.037	0.016	0.382
Number of reflections	28081	1575	1383
<I/σ(I)>	3.4
Completeness [%]	100.0	100	100
Redundancy	38.3	35.5	31.8
CC(1/2)	0.981	0.968	0.778

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	20% (w/v) PEG 3,350, 100 mM PCB buffer pH 4.5