7Y8I

Crystal structure of sDscam FNIII3 domain, isoform alpha7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-04
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97852
Spacegroup name	P 21 21 21
Unit cell lengths	69.113, 85.776, 106.238
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.770 - 1.900
R-factor	0.1987
Rwork	0.198
R-free	0.22940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1va9
RMSD bond length	0.007
RMSD bond angle	0.862
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.930
High resolution limit [Å]	1.900	1.900
Number of reflections	50577	2452
<I/σ(I)>	22.9
Completeness [%]	99.8
Redundancy	12.9
CC(1/2)	0.990	0.665

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.1 M Sodium dihydrogen phosphate pH 6.5, 18% (w/v) PEG 8000, 3% isopropanol