7Y8H

Crystal structure of sDscam FNIII1 domain, isoform alpha7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-04
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97852
Spacegroup name	C 1 2 1
Unit cell lengths	81.977, 43.211, 48.338
Unit cell angles	90.00, 111.29, 90.00

Refinement procedure

Resolution	38.190 - 1.800
R-factor	0.2255
Rwork	0.223
R-free	0.27190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1va9
RMSD bond length	0.007
RMSD bond angle	0.759
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.840
High resolution limit [Å]	1.800	1.800
Number of reflections	14730	939
<I/σ(I)>	13.1
Completeness [%]	99.3
Redundancy	6.2
CC(1/2)	0.970	0.775

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	25% (w/v) PEG 1,500, 100 mM SPG buffer pH 5.5