7Y8D
Crystal structure of cp1 bound BCLxl
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-04-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 69.803, 99.366, 51.359 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.730 - 2.000 |
| R-factor | 0.1903 |
| Rwork | 0.188 |
| R-free | 0.24310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AF2 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.910 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 5.430 | 2.000 |
| Rmerge | 0.070 | 0.029 | 0.663 |
| Rmeas | 0.073 | 0.030 | 0.693 |
| Rpim | 0.020 | 0.008 | 0.197 |
| Total number of observations | 156882 | ||
| Number of reflections | 12248 | 678 | 597 |
| <I/σ(I)> | 7.1 | ||
| Completeness [%] | 98.2 | 98.1 | 95.7 |
| Redundancy | 12.8 | 12 | 11.7 |
| CC(1/2) | 1.000 | 0.907 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 277 | 0.1M HEPES pH7.5, 2.0M Ammonium formate |
