7Y88
class I diterpene synthase (CyS-GGPP-Mg2+) from Streptomyces cattleya
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL02U1 |
Synchrotron site | SSRF |
Beamline | BL02U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-10-23 |
Detector | DECTRIS EIGER2 S 9M |
Wavelength(s) | 0.97918 |
Spacegroup name | F 2 2 2 |
Unit cell lengths | 100.560, 113.137, 138.052 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.753 - 1.870 |
R-factor | 0.1827 |
Rwork | 0.181 |
R-free | 0.20530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7y50 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.570 | 1.910 |
High resolution limit [Å] | 1.870 | 1.870 |
Rmerge | 0.080 | 0.497 |
Number of reflections | 32571 | 3231 |
<I/σ(I)> | 19.7 | |
Completeness [%] | 100.0 | |
Redundancy | 12.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 1000 mM Sodium citrate tribasic and 100 mM Sodium cacodylate / Hydrochloric acid pH 6.5 |