7Y7T
QDE-1 in complex with 12nt DNA template, ATP and 3'-dGTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-01-03 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97928 |
Spacegroup name | P 1 |
Unit cell lengths | 81.505, 85.790, 101.280 |
Unit cell angles | 102.38, 103.41, 94.13 |
Refinement procedure
Resolution | 29.940 - 2.500 |
R-factor | 0.2228 |
Rwork | 0.221 |
R-free | 0.25660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2j7n |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.590 |
High resolution limit [Å] | 2.500 | 5.380 | 2.500 |
Rmerge | 0.120 | 0.084 | 0.450 |
Rmeas | 0.130 | 0.090 | 0.526 |
Rpim | 0.050 | 0.033 | 0.266 |
Total number of observations | 427186 | ||
Number of reflections | 78997 | 8332 | 6251 |
<I/σ(I)> | 8.2 | ||
Completeness [%] | 89.0 | 93.9 | 70.3 |
Redundancy | 5.4 | 7.3 | 2.9 |
CC(1/2) | 0.994 | 0.616 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 291 | peg4000, 200 mM KCl |