7Y7S

QDE-1 in complex with DNA template, RNA primer and AMPNPP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-01-03
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97928
Spacegroup name	P 1 21 1
Unit cell lengths	101.954, 120.412, 114.993
Unit cell angles	90.00, 109.71, 90.00

Refinement procedure

Resolution	30.100 - 2.700
R-factor	0.21
Rwork	0.208
R-free	0.24520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2j7n
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.100	30.000	2.800
High resolution limit [Å]	2.700	5.800	2.700
Rmerge	0.084	0.046	0.449
Rmeas	0.091	0.050	0.494
Rpim	0.034	0.019	0.202
Number of reflections	67239	7232	5026
<I/σ(I)>	6.4
Completeness [%]	94.2	99.4	70.6
Redundancy	6.5	6.8	5.1
CC(1/2)		0.999	0.917

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.3	291	PEG 4000, 200 mM KCl