7Y7R

QDE-1 in complex with DNA template, RNA primer and 3'-dGTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-28
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97928
Spacegroup name	P 1 21 1
Unit cell lengths	101.366, 122.165, 114.921
Unit cell angles	90.00, 109.29, 90.00

Refinement procedure

Resolution	49.570 - 2.100
R-factor	0.2087
Rwork	0.207
R-free	0.24910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2j7n
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.180
High resolution limit [Å]	2.100	4.520	2.100
Rmerge	0.080	0.045	0.446
Rmeas	0.088	0.048	0.544
Rpim	0.036	0.018	0.303
Total number of observations	660472
Number of reflections	143543	15449	11689
<I/σ(I)>	13.1
Completeness [%]	93.5	99.1	76.4
Redundancy	4.6	6.8	2.3
CC(1/2)		0.999	0.251

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.3	291	PEG 4000, 200 mM KCl