7Y7O

Crystal structure of metallo-endoribonuclease YbeY from Staphylococcus aureus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-08-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.28176
Spacegroup name	P 63
Unit cell lengths	100.663, 100.663, 50.187
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.590 - 1.900
R-factor	0.1993
Rwork	0.197
R-free	0.22560
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.899
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.930
High resolution limit [Å]	1.900	1.900
Rmerge	0.056	0.184
Rmeas	0.055	0.194
Rpim	0.012	0.044
Number of reflections	22303	1017
<I/σ(I)>	56	14.6
Completeness [%]	96.6	88.7
Redundancy	21.1	18.8
CC(1/2)	1.000	0.988

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		287.15	0.1 M Sodium citrate (pH 5.5), 40% (v/v) PEG 400, 1 mM Zinc sulfate