7Y6C

Crystal structure of the EscE/EsaG/EsaH complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-14
Detector	DECTRIS PILATUS 300K
Wavelength(s)	0.9791
Spacegroup name	C 1 2 1
Unit cell lengths	123.850, 57.740, 54.810
Unit cell angles	90.00, 98.82, 90.00

Refinement procedure

Resolution	33.318 - 1.400
R-factor	0.2062
Rwork	0.205
R-free	0.23190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7y6b
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.480
High resolution limit [Å]	1.400	1.400
Rmerge	0.053	0.275
Rmeas	0.063	0.322
Rpim	0.033	0.167
Number of reflections	73641	38993
<I/σ(I)>	14.8	4.6
Completeness [%]	98.1
Redundancy	3.6
CC(1/2)	0.996	0.938

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	289.15	3.3 M NaCl, 0.1 M KH2PO4/Na2HPO4