7Y6B
Crystal structure of the EscE/EsaH complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-11-10 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.9791 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 55.550, 52.750, 65.510 |
Unit cell angles | 90.00, 114.03, 90.00 |
Refinement procedure
Resolution | 39.568 - 1.800 |
R-factor | 0.2013 |
Rwork | 0.200 |
R-free | 0.22830 |
Structure solution method | SAD |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.067 | 0.380 |
Rmeas | 0.092 | 0.495 |
Rpim | 0.063 | 0.345 |
Number of reflections | 31956 | 4667 |
<I/σ(I)> | 9 | 2.8 |
Completeness [%] | 99.1 | 99.5 |
Redundancy | 3.5 | 3.6 |
CC(1/2) | 0.995 | 0.868 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 289 | 0.1 M KSCN, 0.1 M Tris pH 8.4, 23% Polyethylene glycol monomethyl ether 2000, 3%(w/v) 1,6-Hexanediol |