7Y62
Crystal structure of human TFEB HLHLZ domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-01-16 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97853 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 54.600, 54.600, 185.300 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.280 - 2.000 |
R-factor | 0.2223 |
Rwork | 0.222 |
R-free | 0.23750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7d8s |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | MOLREP |
Refinement software | PHENIX (1.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.280 | 47.280 | 2.050 |
High resolution limit [Å] | 2.000 | 8.950 | 2.000 |
Rmerge | 0.056 | 0.024 | 1.533 |
Rmeas | 0.058 | 0.025 | 1.573 |
Rpim | 0.014 | 0.006 | 0.352 |
Total number of observations | 417639 | 4585 | 32151 |
Number of reflections | 22446 | 322 | 1628 |
<I/σ(I)> | 27.2 | 68 | 2.1 |
Completeness [%] | 99.2 | 99.9 | 100 |
Redundancy | 18.6 | 14.2 | 19.7 |
CC(1/2) | 1.000 | 1.000 | 0.918 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | Ethylene glycols, Buffer system 3, and Precipitant Mix 2 |