7Y5K

Crystal structure of human CAF-1 core complex in spacegroup C2221

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-01-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97853
Spacegroup name	C 2 2 21
Unit cell lengths	108.870, 169.843, 153.314
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.330 - 3.480
R-factor	0.2285
Rwork	0.225
R-free	0.25740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3gfc 3fm0
RMSD bond length	0.001
RMSD bond angle	0.449
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.20_4459: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.630
High resolution limit [Å]	3.480	3.500
Rmerge	0.109	0.750
Number of reflections	18554	1833
<I/σ(I)>	19.1
Completeness [%]	99.9
Redundancy	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	7% Tacsimate, pH 6.0, 18% (w/v) PEG 3350, 0.12 M lithium citrate and 0.13 M sodium tartrate