7Y5F
Crystal structure of CmnC in complex with L-homoarginine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-07-31 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9732 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 93.344, 127.514, 139.034 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.890 - 1.520 |
| R-factor | 0.1811 |
| Rwork | 0.180 |
| R-free | 0.20370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7vgl |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.385 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.580 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.035 | 0.369 |
| Number of reflections | 123057 | 11857 |
| <I/σ(I)> | 31.9 | 2.79 |
| Completeness [%] | 98.8 | 95.5 |
| Redundancy | 4.5 | 4.4 |
| CC(1/2) | 0.928 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.2M Ammonium acetate, 0.1M Na citrate tribasic dihydrate pH 5.5, 24% v/v Polyethylene glycol 400 |
