7Y4X

Crystal structure of sDscam Ig1 domain, isoform alpha7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97855
Spacegroup name	P 43 21 2
Unit cell lengths	92.530, 92.530, 69.884
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.714 - 2.952
R-factor	0.2122
Rwork	0.210
R-free	0.24570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6zr7
RMSD bond length	0.003
RMSD bond angle	0.757
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.000
High resolution limit [Å]	2.950	8.000	2.950
Rmerge	0.209	0.162	1.420
Rmeas	0.214	0.166	1.462
Rpim	0.043	0.035	0.345
Total number of observations	164824
Number of reflections	6793	403	329
<I/σ(I)>	4.5
Completeness [%]	99.9	99.8	99.4
Redundancy	24.3	21.6	17.4
CC(1/2)	0.965	0.993	0.741

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.2 M Ammonium acetate, 0.1 M Sodium citrate tribasic dihydrate pH 6.0, 25% PEG 3,350, 5% Jeffamine M-600 pH7