7Y4X
Crystal structure of sDscam Ig1 domain, isoform alpha7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-12-05 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97855 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 92.530, 92.530, 69.884 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.714 - 2.952 |
R-factor | 0.2122 |
Rwork | 0.210 |
R-free | 0.24570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6zr7 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.757 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.15.2_3472: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.000 |
High resolution limit [Å] | 2.950 | 8.000 | 2.950 |
Rmerge | 0.209 | 0.162 | 1.420 |
Rmeas | 0.214 | 0.166 | 1.462 |
Rpim | 0.043 | 0.035 | 0.345 |
Total number of observations | 164824 | ||
Number of reflections | 6793 | 403 | 329 |
<I/σ(I)> | 4.5 | ||
Completeness [%] | 99.9 | 99.8 | 99.4 |
Redundancy | 24.3 | 21.6 | 17.4 |
CC(1/2) | 0.965 | 0.993 | 0.741 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.2 M Ammonium acetate, 0.1 M Sodium citrate tribasic dihydrate pH 6.0, 25% PEG 3,350, 5% Jeffamine M-600 pH7 |