7Y4J

HapR_Triple mutant Y76F, L97I, F171C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER X8 PROTEUM
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-12
Detector	Bruker PHOTON III
Wavelength(s)	1.54
Spacegroup name	P 21 21 2
Unit cell lengths	88.587, 43.439, 53.326
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.326 - 2.690
R-factor	0.243
Rwork	0.237
R-free	0.28780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7xy5
Data reduction software	PROTEUM PLUS
Data scaling software	PROTEUM PLUS
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.326	2.790
High resolution limit [Å]	2.690	2.690
Number of reflections	11033	588
<I/σ(I)>	7
Completeness [%]	100.0	100
Redundancy	7.2
CC(1/2)	0.980	0.550

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.5	295	7-8% PEG 8000, 100mM CHES 9.5, 200mM NaCl