7Y4I
Crystal structure of SPINDLY in complex with GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2021-11-01 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 127.735, 130.677, 141.368 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.340 - 2.850 |
| R-factor | 0.2158 |
| Rwork | 0.213 |
| R-free | 0.26990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AF2D |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.613 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 65883 | 6457 |
| <I/σ(I)> | 8.1 | |
| Completeness [%] | 99.6 | 99.6 |
| Redundancy | 13 | 13 |
| CC(1/2) | 1.000 | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7.5 | 289 | 0.1 M bicine, 24% Polyethylene glycol 1,500, and 10% (v/v) 2-Propanol |
