7Y4D
Crystal structure of DUSP10 mutant_S95A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2022-02-22 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 1 |
Unit cell lengths | 39.270, 42.649, 56.526 |
Unit cell angles | 74.32, 82.35, 63.86 |
Refinement procedure
Resolution | 24.970 - 2.180 |
R-factor | 0.185 |
Rwork | 0.180 |
R-free | 0.23000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zzw |
RMSD bond length | 0.013 |
RMSD bond angle | 1.362 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.260 |
High resolution limit [Å] | 2.180 | 2.180 |
Rmerge | 0.023 | 0.139 |
Number of reflections | 15595 | 15568 |
<I/σ(I)> | 34.111 | 5.264 |
Completeness [%] | 94.3 | |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 283 | 22.5% (w/v) PEG 3000, 100 mM Tris base /Hydrochloric acid, 200 mM Calcium acetate |