7Y3M
Structure of SALL4 ZFC1 bound with 16 bp AT-rich dsDNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-12-10 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 74.587, 65.706, 102.980 |
Unit cell angles | 90.00, 106.78, 90.00 |
Refinement procedure
Resolution | 33.789 - 2.723 |
R-factor | 0.2266 |
Rwork | 0.223 |
R-free | 0.26820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7y3i |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 67.890 | 2.870 |
High resolution limit [Å] | 2.720 | 2.720 |
Number of reflections | 25181 | 25181 |
<I/σ(I)> | 15.8 | |
Completeness [%] | 98.6 | |
Redundancy | 6.7 | |
CC(1/2) | 0.999 | 0.930 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.1M HEPES, pH 6.5, 10% polyethylene glycol 6000, 5% (v/v) 2-Methyl-2,4-pentanediol (MPD) |