7Y3C

Crystal structure of TRIM7 bound to RACO-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2021-11-06
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979183
Spacegroup name	P 65
Unit cell lengths	79.898, 79.898, 53.108
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.950 - 1.710
R-factor	0.1644
Rwork	0.163
R-free	0.19760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6uma
RMSD bond length	0.007
RMSD bond angle	0.965
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.190	1.800
High resolution limit [Å]	1.710	1.710
Rmerge	0.150	1.506
Number of reflections	19719	2813
<I/σ(I)>	12.9
Completeness [%]	93.5
Redundancy	15.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	0.2 M Potassium sodium tartrate tetrahydrate, 26% w/v Polyethylene glycol 3,350