7Y3A
Crystal structure of TRIM7 bound to 2C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NFPSS BEAMLINE BL17B |
Synchrotron site | NFPSS |
Beamline | BL17B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2021-09-20 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 79.228, 53.644, 80.999 |
Unit cell angles | 90.00, 119.21, 90.00 |
Refinement procedure
Resolution | 29.040 - 1.700 |
R-factor | 0.1496 |
Rwork | 0.148 |
R-free | 0.17510 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6uma |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.700 | 1.800 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.046 | 0.140 |
Number of reflections | 61973 | 3432 |
<I/σ(I)> | 23.73 | |
Completeness [%] | 99.4 | |
Redundancy | 12.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Sodium formate pH 7.0 20%3350 0.2M NaCl |