7Y39

Ubiquitin-like domain of human ZFAND1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2020-05-22
Detector	ADSC QUANTUM 210r
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	49.158, 34.284, 64.522
Unit cell angles	90.00, 91.97, 90.00

Refinement procedure

Resolution	23.490 - 1.880
R-factor	0.2073
Rwork	0.205
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	AF-Q8TCF1-F1
Data scaling software	HKL-2000
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.20-4444)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.950
High resolution limit [Å]	1.880	4.050	1.880
Rmerge	0.048	0.042	0.244
Rmeas	0.059	0.052	0.290
Rpim	0.033	0.030	0.155
Total number of observations	55773
Number of reflections	17016	1781	1665
<I/σ(I)>	12.5
Completeness [%]	96.0	96.1	96.3
Redundancy	3.3	3.2	3.3
CC(1/2)		0.996	0.976

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1M MES (pH6.5), 1.2M Sodium Citrate