7Y2D
HSA-Cu agent complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-01-16 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.987 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 186.330, 38.830, 95.000 |
| Unit cell angles | 90.00, 105.66, 90.00 |
Refinement procedure
| Resolution | 30.062 - 2.000 |
| R-factor | 0.2354 |
| Rwork | 0.233 |
| R-free | 0.28610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5yb1 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.101 |
| Data reduction software | HKL-3000 (7.21) |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.090 | 2.180 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.061 | 0.061 |
| Number of reflections | 44865 | 9780 |
| <I/σ(I)> | 17.7 | |
| Completeness [%] | 99.7 | |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG 3350, MPD, DMSO |
