7Y1S

Crystal structure of apo leucyl aminopeptidase from Bacillus amyloliquefaciens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-19
Detector	DECTRIS PILATUS3 S 2M
Wavelength(s)	0.9793
Spacegroup name	P 63 2 2
Unit cell lengths	200.245, 200.245, 89.022
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.871 - 2.748
Rwork	0.170
R-free	0.21180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3jru
RMSD bond length	0.009
RMSD bond angle	1.855
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.848
High resolution limit [Å]	2.748	2.748
Number of reflections	27747	2734
<I/σ(I)>	21.69
Completeness [%]	99.2
Redundancy	30.3
CC(1/2)	0.996	0.970

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	0.5 M succinic acid (pH 7.0), 0.1 M Bis-Tris propane (pH 7.0)