7Y1P

CRYSTAL STRUCTURE OF THE RGS-HOMOLOGOUS DOMAIN OF AXIN IN COMPLEX WITH LZ-22Na

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	193.15
Detector technology	PIXEL
Collection date	2021-11-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	I 4
Unit cell lengths	155.343, 155.343, 107.061
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.836 - 2.602
R-factor	0.2089
Rwork	0.208
R-free	0.22750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dk8
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3247)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.640
High resolution limit [Å]	2.600	7.050	2.600
Rmerge	0.100	0.038	0.895
Rmeas	0.108	0.042	0.969
Rpim	0.041	0.016	0.369
Total number of observations	265978
Number of reflections	39114	2023	1950
<I/σ(I)>	4.7
Completeness [%]	100.0	99.8	100
Redundancy	6.8	6.5	6.8
CC(1/2)		0.999	0.768

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291.15	2.4 M sodium malonate pH 7.0