7Y17
Crystal structure of ribosomal ITS2 pre-rRNA processing complex from Cyberlindnera jadinii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-01-12 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 152.619, 240.021, 237.003 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.410 - 3.390 |
| R-factor | 0.3099 |
| Rwork | 0.309 |
| R-free | 0.33190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6of4 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.787 |
| Data reduction software | HKL-3000 (7.21) |
| Data scaling software | HKL-3000 (7.21) |
| Phasing software | PHENIX (1.17.1_3660) |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.290 |
| High resolution limit [Å] | 3.230 | 3.230 |
| Rpim | 0.135 | 0.412 |
| Number of reflections | 41321 | 3403 |
| <I/σ(I)> | 4.3 | |
| Completeness [%] | 99.9 | |
| Redundancy | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.1 M sodium phosphate, pH 7.5, 0.05 M NaCl, and 9% PEG 4000 |
