7Y0Y
Crystal structure of Pseudomonas aeruginosa PvrA (SeMet)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-06-30 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97852 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 93.026, 97.609, 105.443 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.880 - 2.090 |
R-factor | 0.2059 |
Rwork | 0.203 |
R-free | 0.25010 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 0.919 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.140 |
High resolution limit [Å] | 2.090 | 2.090 |
Rpim | 0.044 | 0.209 |
Number of reflections | 28197 | 2708 |
<I/σ(I)> | 27 | |
Completeness [%] | 100.0 | |
Redundancy | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 25% w/v PEG 3350 |