7Y0V
The co-crystal structure of BA.1-RBD with Fab-5549
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL10U2 |
Synchrotron site | SSRF |
Beamline | BL10U2 |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2022-03-06 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 64.770, 138.910, 178.050 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.090 - 2.480 |
R-factor | 0.2148 |
Rwork | 0.211 |
R-free | 0.26760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7e88 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.090 | 2.540 |
High resolution limit [Å] | 2.480 | 2.480 |
Rmerge | 0.104 | 1.668 |
Number of reflections | 28861 | 2821 |
<I/σ(I)> | 12.98 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 12.4 | 11.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.25 | 291 | 0.1 M HEPES sodium pH 7.25, 10% v/v 2-Propanol, 18% w/v Polyethylene glycol 4,000 |