7Y0G

Crystal structure of anti-mPEG h15-2b Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	80
Detector technology	CCD
Collection date	2020-06-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9732
Spacegroup name	P 1 21 1
Unit cell lengths	63.994, 112.285, 158.730
Unit cell angles	90.00, 90.58, 90.00

Refinement procedure

Resolution	30.000 - 2.080
R-factor	0.2126
Rwork	0.211
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1l7i
RMSD bond length	0.005
RMSD bond angle	1.359
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.150
High resolution limit [Å]	2.080	2.080
Rmerge	0.058	0.492
Number of reflections	131932	12417
<I/σ(I)>	24.77	2.68
Completeness [%]	98.5	93.1
Redundancy	4.8	4.4
CC(1/2)		0.864

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	18% (w/v) PEG-6000, 1% (w/v) PEG-2000-MME, 0.15 M lithium sulfate monohydrate, and 0.1 M citric acid