7XZC
Mutant (R240A) of the N-terminal domain of fucoidan lyase FdlA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 193.15 |
| Detector technology | PIXEL |
| Collection date | 2020-11-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 |
| Unit cell lengths | 53.798, 57.972, 88.150 |
| Unit cell angles | 90.23, 78.68, 87.86 |
Refinement procedure
| Resolution | 47.860 - 1.700 |
| R-factor | 0.1649 |
| Rwork | 0.164 |
| R-free | 0.19180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7xzf |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.092 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.770 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmeas | 0.090 | 0.660 |
| Number of reflections | 107712 | 5947 |
| <I/σ(I)> | 7.2 | |
| Completeness [%] | 94.9 | 94.7 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291.15 | 0.1 M HEPES sodium pH 7.5, 10% (v/v) 2-Propanol, 20% PEG 4000 |
