7XZA
Mutant (Y242F) of the N-terminal domain of fucoidan lyase FdlA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL02U1 |
Synchrotron site | SSRF |
Beamline | BL02U1 |
Temperature [K] | 193.15 |
Detector technology | PIXEL |
Collection date | 2021-10-03 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 |
Unit cell lengths | 54.068, 58.150, 88.164 |
Unit cell angles | 90.16, 101.69, 92.11 |
Refinement procedure
Resolution | 27.750 - 2.080 |
R-factor | 0.1757 |
Rwork | 0.173 |
R-free | 0.22670 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7xzf |
RMSD bond length | 0.008 |
RMSD bond angle | 0.955 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 86.000 | 2.190 |
High resolution limit [Å] | 2.080 | 2.080 |
Rmeas | 0.170 | 0.336 |
Number of reflections | 57344 | 8676 |
<I/σ(I)> | 3.9 | |
Completeness [%] | 91.0 | |
Redundancy | 1.7 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 10.5 | 291.15 | 0.1 M CAPS pH 10.5, 0.2 M lithium sulfate, 2 M ammonium sulfate |